Part I: In Vitro Toxicological High-Thoughput Screening Methods

1. Monitoring Ligand-Activated Protein-Protein Interactions Using Bioluminescent Resonance Energy Transfer (BRET) Assay

   Carlos Coriano, Emily Powell, and Wei Xu

2. Mitochondrial Membrane Potential Assay

   Srilatha Sakamuru, Matias S. Attene-Ramos, and Menghang Xia

3. Using beta-Lactamase and NanoLuc Luciferase Reporter Gene Assays to Identify Inhibitors of the HIF-1 Signaling Pathway Thai Khuc, Chia-Wen (Amy) Hsu, Srilatha Sakamuru, and Menghang Xia

4. Quantitative High-Throughput Luciferase Screening in Identifying CAR Modulators

   Caitlin Lynch, Jinghua Zhao, Hongbing Wang, and Menghang Xia

5. Transactivation and Coactivator Recruitment Assays for Measuring Farnesoid X Receptor Activity

   Chia-Wen (Amy) Hsu, Jinghua Zhao, and Menghang Xia

6. Cell-Based Assay for Identifying the Modulators of Antioxidant Response Element Signaling Pathway

   Jinghua Zhao, Sunita J. Shukla, and Menghang Xia

7. Study Liver Cytochrome P450 3A4 Inhibition and Hepatotoxicity Using DMSO-Differentiated HuH-7 Cells

   Yitong Liu

8. Determination of Histone H2AX Phosphorylation in DT40 Cells

   Kana Nishihara, Sampada A. Shahane, and Menghang Xia

9. High-Throughput and High Content Micronucleus Assay in CHO-K1 Cells Sampada A. Shahane, Kana Nishihara, and Menghang Xia

Part II: In Vivo Toxicological High-Thoughput Screening Methods

1. Better, Faster, Cheaper: Getting the Most Out of High-Throughput Screening with Zebrafish

Lisa Truong, Michael T. Simonich, and Robert L. Tanguay

1. Fast Functional Germline and Epigenetic Assays in the Nematode Caenorhabditis elegans

Zachary Lundby, Jessica Camacho, and Patrick Allard

Part III: In Silico High-Thoughput Screening Toxcity Data Analysis

1. A Quantitative High-Throughput Screening Data Analysis Pipeline for Activity Profiling

Ruili Huang

1. Correction of Microplate Data from High-Throughput Screening

Yuhong Wang and Ruili Huang

1. CurveP Method for Rendering High-Throughput Screening Dose-Response Data into Digital Fingerprints

Alexander Sedykh

1. Accounting Artifacts in High-Throughput Toxicity Assays

Jui-Hua Hsieh

1. Accessing the High-Throughput Screening Data Landscape

Daniel P. Russo and Hao Zhu

1. Curating and Preparing High Throughput Screening Data for Quantitative Structure Activity Relationship Modeling

Marlene T. Kim, Wenyi Wang, Alexander Sedykh, and Hao Zhu