1. Introduction to FOXO Biology

Wolfgang Link

Part I: Biochemical and Molecular Methods

1. Characterization of microRNAs Regulating FOXO Expression

Doug Hanniford and Eva Hernando

1. Identification of Transcription Factor Binding Sites in the Mouse FoxO1 Promoter

Christopher P. Cardozo

1. Investigating Mechanisms that control Ubiquitin-mediated DAF-16/FOXO Protein Turnover

Thomas Heimbucher and Coleen T. Murphy

1. Phosphorylation of FOXO Proteins as a Key Mechanism to Regulate their Activity

Alba Orea-Soufi, David Dávila, María Salazar-Roa, María de Mar Lorente, and Guillermo Velasco

1. Methodological Approach for the Evaluation of FOXO as a Positive Regulator of Antioxidant Genes

María Monsalve, Ignacio Prieto, Andreza Fabro de Bem, and Yolanda Olmos

1. Characterization of FOXO Acetylation

Shang Yao, Zimam Mahmud, Nikoleta Sachini, Sathid Aimjongjun, Paula Saavedra-García, and Eric W-F Lam

1. Transcriptional Activity of FoxO Transcription Factors Measured by Luciferase Assays

Antonia Ávila-Flores, Javier Arranz-Nicolás, and Isabel Mérida

1. Monitoring the Transcriptional Activity of FOXO Transcription Factors by Analyzing their Target Genes

Vanessa Henriques, Susana Machado, Wolfgang Link, and Bibiana I. Ferreira

1. Using ChIP-based Approaches to Characterize FOXO Recruitment to its Target Promoters

Neeraj Kumar and Arnab Mukhopadhyay

1. RNAi Mediated Silencing of FoxO Factors

Priyankar Sanphui, Akash Saha, and Subhas Chandra Biswas

Part II: Imaging Approaches

1. Immunofluorescence Analysis by Confocal Microscopy for Detecting Endogenous FOXO

Francisco Castillo, Thomas A. Mackenzie, and Bastien Cautain

1. High Throughput Image-based Screening to Identify Chemical Compounds Capable of Activating FOXO

Marta Barradas, Wolfgang Link, Diego Megías, and Pablo J. Fernández-Marcos

1. Image-based Identification of Chemical Compounds Capable of Trapping FOXO in the Cell Nucleus

Susana Machado, Catarina Raposo, Bibiana I. Ferreira, and Wolfgang Link

1. Quantifying Tissue-specific Overexpression of FOXO in Drosophila via mRNA Fluorescence in situ Hybridization using Branched DNA Probe Technology

Anna C Blice-Baum, Georg Vogler, Meera C Viswanathan, Bosco Trinh, Worawan B Limpitikul, and Anthony Cammarato

Part III: Omics and Bioinformatic Approaches

1. Genome-wide Analysis for Identifying FOXO Protein Binding Sites

Dong-Ju Shin, Pujan Joshi, Dong-Guk Shin, and Li Wang

1. Mathematical Modeling of Nuclear Trafficking of FOXO Transcription Factors

Bradford E. Peercy and Martin F. Schneider

1. Following Transcriptome to Uncover FOXO Biological Functions

Raymond Liang, Vijay Menon, and Saghi Ghaffari

Part IV: Animal Models

1. Hydra as Model to Determine the Role of FoxO in Longevity

Thomas C. G. Bosch

1. Genetic Ablation of FOXO in Mice to Investigate its Physiological Role

Hwanhee Oh and Jihye Paik

Part V: Human Gentic and Clinical Studies

1. Analysis of FOXO3 Gene Polymorphisms Associated with Human Longevity

Timothy A Donlon, Philip MC Davy, and Bradley J Willcox

1. Analysis of FOXO3a Gene Polymorphism Associated with Asthma

Tejas Shah and Kalpana Joshi