Part I: Properties, Design, Synthesis and Characterization of AMPs

1. Antimicrobial Peptides: An Introduction

Evan F. Haney, Sarah C. Mansour, and Robert E.W. Hancock

1. Tools for Designing Amphipathic Helical Antimicrobial Peptides

Davor Juretic, Damir Vukicevic, and Alessandro Tossi

1. Chemical Synthesis of Antimicrobial Peptides

Lena Münzker, Alberto Oddo and Paul R. Hansen

1. Microwave-Assisted Synthesis of Antimicrobial Peptides

Suhas Ramesh, Beatriz G. de la Torre, Fernando Albericio, Hendrik G. Kruger, and Thavendran Govender

1. High Performance Liquid Chromatography and Mass Spectrometry-Based Design of Protolytically Stable Antimicrobial Peptides

Mojtaba Bagheri and Robert E. W. Hancock

1. Determination of Structure and Micellar Interactions of Small Antimicrobial Peptides by Solution-State NMR

Reinhard Wimmer and Lars Erik Uggerhøj

1. Mass Spectrometry Approaches for Determining the Structure of Antimicrobial Peptides

Jiongyu Liu and Jianping Jiang

Part II: Studying Interactions of AMPs with Model Membranes and Bacteria

1. Molecular Dynamics Simulation and Analysis of the Antimicrobial Peptides-Lipid Bilayers Interactions

Shima Arasteh and Mojtaba Bagheri

1. Calorimetry Methods to Study Membrane Interactions and Perturbations Induced by Antimicrobial Host Defense Peptides

Mauricio Arias, Elmar J. Prenner and Hans J. Vogel

1. Fluorescence and Absorbance Spectroscopy Methods to Study Membrane Perturbations by Antimicrobial Host Defense Peptides

Mauricio Arias and Hans J. Vogel

1. Applying Fluorescence Correlation Spectroscopy to Investigate Peptide-Induced Membrane Disruption

Kasper Kristensen, Jonas R. Henriksen, and Thomas L. Andresen

1. Macromolecule Biosynthesis Assay and Fluorescence Spectroscopy Methods to Explore Antimicrobial Peptide Mode(s) of Action

Bimal Jana, Kristin Renee Baker, and Luca Guardabassi

1. Using Confocal Microscopy and Computational Modeling to Investigate the Cell Penetrating Properties of Antimicrobial Peptides

Gabriel Del Rio, Edda Klipp, and Andreas Herrmann

1. Atomic Force Microscopy Study of the Interactions of Indolicidin with Model Membranes and DNA

Peter Fojan and Leonid Gurevich

1. Protocols for studying the interaction of MSI-78 with the Membranes of Whole Gram-positive and Gram-negative Bacteria by NMR

Nury P. Santisteban , M. R. Morrow, and V. Booth

1. Preparation of membrane models of Gram-negative bacteria and their interaction with Antimicrobial Peptides studied by CD and NMR

Rickey P. Hicks

1. Studying the Interaction of Magainin 2 and Cecropin A with E. coli Bacterial Cells using Circular Dichroism

Concetta Avitabile, Luca Domenico D'Andrea and Alessandra Romanelli

1. Studying the Mechanism of Membrane Permeabilization induced by Antimicrobial Peptides using Patch-clamp Techniques

Giorgio Rispoli

1. Assays for Identifying Inducers of the Antimicrobial Peptide LL-37

Frank Nylen, Peter Bergman, Gudmundur H. Gudmundsson, and Birgitta Agerberth

1. Methods for Elucidating the Mechanism of Action of Proline-rich and other Non-lytic Antimicrobial Peptides Monica Benincasa, Giulia Runti, Mario Mardirossian, Renato Gennaro,and Marco Scocchi

2. The Interaction of Antimicrobial Peptides with the Membrane and Intracellular Targets of Staphylococcus Aureus Investigated by ATP-leakage, DNA-binding Analysis and the Expression of a LexA Controlled Gene, recA

Sanne Gottschalk and Line Elnif Thomsen

Part III: Assaying Selected Biological Activities of AMPs

1. Methods for Investigating Biofilm Inhibition and Degradation by Antimicrobial Peptides

Li-av Segev-Zarko and Yechiel Shai

1. Protocols for Studying Inhibition and Eradication of Bacterial Biofilms by Antimicrobial Peptides

Vijayalekshmi Sarojini

1. Protocols for Studying Antimicrobial Peptides (AMPs) as Anti-cancer Agents

Laurence Madera and David W. Hoskin

1. Using Oral a